The cord blood mononuclear cells(CBMNCs) were separated by density gradient centrifugation and cultured in presence of relevant cytokines(rhGM-CSF,rhIL-4 and rhTNF-α)to generate DCs in order to explore the killing effect of CTL induced by human cord blood dendritic cells(DCs) loaded with mild acid-eluted peptides of esophageal carcinoma cells on cytotoxicity of T lymphocyte.The specific CTLs were induced by DCs loaded with mild acid-eluted peptides of esophageal carcinoma cell line T.Tn.The killing effect of CTLs on T.Tn.target cells was identified by CytoTox96 assay in vitro.The CTLs induced by DCs loaded with antigen peptides exhibit significant killing activity to T.Tn cells before mild acid-eluted(78.5±9.5)% compared to the cells after mild acid-eluted(P<0.05).It is higher than Hep2 cell line and the controls(P<0.05).The specific killing activity of CTLs stimulated by cord blood DCs loaded with antigen peptides can be induced.It provides a potent method to the immunotherapy of esophageal carcinoma.