The previous batch experiments have verified that three kinds of aquatic plants(Myriophyllum,Ceratophyllum and Potamogeton pectinatus)can obviously absorb and decompose the nutrients and pollutants in water body. In order to further verify the treatment effect of the selected aquatic plants' combination on the actual polluted water,a plant fence was established to study the removal of the nutrients in polluted water under in-situ conditions. The results showed that the combination of aquatic plants in the fence could remove the pollutant in water under in-situ conditions. Compared to the control group,the combination of Myriophyllum and Ceratophyllum showed the best removal effect of nitrogen nutrients,the combination of Potamogeton pectinatus and Myriophyllum showed the best removal effect of dissolved phosphorus nutrients in the water,while the combination of grate tooth eye and the fox tail algae showed the best removal effect of the dissolved COD_Mn. In a comprehensive view,the combination of the Fox tail algae + Ceratophyllum is the best for the removal of nutrients.