In order to explore the deep denitrification efficiency of sulfur autotrophic denitrifying filter for reclaimed water from municipal sewage treatment plant, this study takes Handan East Sewage Treatment Plant as the research object adopting a pilot experiment to simulate the actual production situation, meanwhile under the experiment to analyze the pilot operation problems and calculate the operating cost. The result shows:the treatment scale of test system is 200 m³/d; the direct operating cost is 0.13 yuan/m³; the nitrate nitrogen removal effect is significant as the removal rate is above 98%; This pilot-scale experiment can effectively reduce the total nitrogen in the effluent, without affecting other effluent indicators.