Kinesin is a kind of molecule motor that moves along the microtubule,and the study of its movement mechanism will help human reveal the energy conversion mechanism of the life systems.In this paper,based on the crystal structure of a kinesin motor and simulated by solving Poisson's equation,the electrostatic interactions of kinesin along the microtubule have been calculated,the results show that the electrostatic interactions play an important role when kinesin moves along microtubule,and the interaction periodicity is consistent with the periodicity of microtubule,then we present the mechanism of kinesin under the electrostatic interaction.