Abstract: Teacher-student relationship is not only the basic human relation in university, but also the important relation in the process of education. As post-80's, young teachers has become the main force of frontline teachers in education, and post-90s become the main body of university students, it is more and more important to deeply study the construction of harmonious relationship between the two generations. This paper expounds the advantages and problems of post-80's young teachers, analyzes the features of post-90's university students, and makes suggestions for the construction of harmonious relationship between young teachers and students in university from classroom teaching, daily life and internet world.