This paper discusses the cooperation motivation mechanism, restriction mechanism and incentive mechanism between colleges and enterprises. The essence of the cooperation between colleges and enterprises is a double subject seeking "win-win" result of the collaborative innovation process. It is a fusion of the advantages of the both. In the process of cooperation, Different parties play different roles:Due to the degree of participation, the role of industry enterprises is different. The cooperation has one center and two bases. The center should be the demand of enterprises. It should begin with the analysis of enterprise demands and end with track. And the cooperation things should be related with teacher evaluation in order to motivating teachers' cooperation, setting up the teaching management system based on the combination of work and study.