Based on the important role of discipline competition in cultivating innovative and entrepreneurial talents, this article expounds some concrete methods and measures of constructing education system of innovation and entrepreneurship based on discipline competition in our university according to the establishment of discipline competition system, competition team, course system, engineering training system, organizational management system and evaluation system. By incorporating the discipline competition into the normal teaching plan, a relatively complete innovation and entrepreneurship education system and a good education teaching order have been formed. So a strong guarantee is provided for the cultivation of innovative and entrepreneurial talents.