The application of innovative teaching mode in College English micro teaching can effectively improve the quality of College English teaching. Firstly, this paper expounds the theory and teaching function of micro-lesson, then illustrates the application significance of micro-lesson in College English teaching. Then, combining with practical examples, it analyzes and studies a kind of flipped classroom teaching mode based on micro-lesson, hoping that this paper can play a positive role in the innovation of College English teaching mode in China.