In accordance with the concept of Ecological education, and based on the analyses of domestic innovation and entrepreneurship education system, combining the practical experience of teaching the subject, we put forward the innovation and entrepreneurship education system of the "five-in-one", including innovation and entrepreneurship, career education, employment education, professional education, ideological and political education. The construction, operating mechanism and practical operation of the system are also elaborated. This research aims to improve the mode of teaching of theory and practice of innovation and entrepreneurship education in colleges and universities, and enhance the quality of education of innovation and entrepreneurship.