As the soul of the development in disciplines, discipline culture works as the spiritual source and internal motivation for the continuous development and innovation of the disciplines. Discipline cultural self-confidence is an important part of cultural self-confidence. While Cultivating college students' cultural self-confidence, the overall situation should be focused on for promotion as a whole. Disciplinary cultural self-confidence share unity with cultural self-confidence and is isomorphic with the cultivation of core values, and are generative with the role of national rejuvenation. To cultivate the confidence of college students in discipline culture, we must deeply dig and integrate the connotation of the discipline culture, constantly innovate and develop cultivating methods, and finally reach the goal of infiltrating and shaping the college students' confidence of the new era. The cultivation of confidence in discipline culture can achieve the effect of stimulating students' academic enthusiasm, promoting the comprehensive development of students and disciplines and the core values of socialism, firming ideals and beliefs, and fulfilling the mission to practice.