The political leadership of the rural grass-roots party organization is the manifestation of the political leadership of the Party at the grass-roots level, and is the political guarantee to lead the farmers to realize rural revitalization and rural modernization. Therefore, to improve the political orientation, competence, execution and influence of the rural grass-roots party organization is an inevitable requirement to enhance its political leadership. On the basis of the actual construction of rural grassroots party organizations, this paper, taking villages S and X in the north of Anhui as examples, has found that there are still some constraints for rural grassroots party organizations in the process of political leadership promotion, such as leadership role to be strengthened, organizational structure to be improved, and inner-party care mechanism to be improved. Therefore, based on these facts, this paper views that the political leadership of rural grassroots party organization should be enhanced from three aspects: highlighting the leading role of organizations, strengthening the team construction of agricultural organizations, and improving the inner-party care mechanism.